The best VSV-G monoclonal antibody?

One of the most frustrating things in the lab is spending good money on an antibody only to find out it doesn’t work well in your application. When you try to find a VSV-G antibody, besides host and application concerns, the clonality of antibody would be another key concern candidate.

Both polyclonal and monoclonal antibodies each have their own advantages which make them useful for different applications, while monoclonal antibodies are much more specific and with less background noise than polyclonal antibodies, and are as such generally preferable for biochemical assays.

VSV-G monoclonal antibody vs. VSV-G polyclonal antibodies

Currently there’re very limited source of VSV-G monoclonal antibodies in the market. One is from Sigma with clone # P5D4, another is from Abbkine with clone #14D2. Now let start VSV-G Monoclonal Antibody introduction from Sigma.

The VSV-G antibody from Sigma, clone number P5D4, recognizes an epitope containing the five C-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell epipolic and in the budding virus. Due to aquisition of Sigma by Merck-Millipore, Merck-Millipore would suply this antibody from 2016 in your region.

As you know or may not know, one manufacturer may license their antibody to other companies, so do not buy three antibodies to test without making sure they are actually different. With common monoclonals that are supplied by many companies you might also consider shopping around for the best supplier of the target protein. Abbkine is such of OEM supplier with develop and produce full portfolio of loading control antibodies and tag antibodies, including His, HA, Flag, MBP, mCherry, GST, GFP, V5, VSV-G, Digoxin, Ubiquitin, BGF, RFP,mOrange, AmCyan, T7, Strep, S tag, KT3, etc, which have been commonly used around world.

Here, we combine Abbkine’s high quality VSV-G Tag Mab with Sigma VSV-G antibody together for your brief comparison.

Vendor Clone # Applications Price Yr2017
Sigma VSV-G antibody P5D4 WB, IF, IP $340/100μl
Abbkine Vs. Sigma VSV-G antibody 14D2 WB, IF, IP $90/50μl

The Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) from Abbkine was affinity-purified from mouse ascites by affinity-chromatography using synthetic peptide immunogen containing the 15 carboxy-terminal amino acids (497-511) of VSV-G conjugated to KLH. It featured multiple applications, including WB, IF and IP with high antibody tier.

Super replacement for Sigma VSV-G antibody

Abbkine VSV-G-Tag Mouse Monoclonal Antibody (14D2) performance in IP (1:200) – WB (1:5,000) analysis of VSV-G Tag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with VSV-G tag protein (lane B); IP untransfected 293 cell lysate with Anti VSV-G tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti VSV-G tag mAb (lane E).

Super replacement for Sigma VSV-G antibody

Abbkine VSV-G-Tag Mouse Monoclonal Antibody (14D2) performance in Immunofluorescence staining (1:1,000) of VSV-G fusion protein in 293 cells with red and counterstained with DAPI.

So now you have two choice to get your VSV-G monoclonal antibody. If you’re willing to saving cost or only need small size, Abbkine would be yours.

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