Tag Archives: VSV-G Tag

Choose right VSV-G Tag Antibody?

Epitope tags include VSV-G tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Choose right VSV-G Tag Antibody?If you’re working in the life sciences with VSV-G tag in your vector or fusion proteins, chances are you’re going to be using right VSV-G antibodies in your research. Here we’ve set out some top tips to get you started in making a good and suitable VSV-G tag antibody buying decision.

Is this VSV-G antibody suitable for your application?

It’s “impossible” to predict which antibodies will work and which will not.  Just because an antibody has been successfully used in one application does not mean it will work for all applications. That means excellent WB and IHC results do not tell you whether the antibody is the right choice for a so-called ChIP-seq experiment, in which chromatin immunoprecipitation is followed by second-generation sequencing.

You must choose application-specific validation of VSV-G antibody you use. However, due to the price and performance, researchers prefer the antibody with more validated applications, especially these kinds of epitope antibodies.

Decision on monoclonal or polyclonal VSV-G tag antibody

Our advice is you should go with the most validated antibody, but if you are lucky enough to have two well validated antibodies to chose between, then polyclonal antibodies tend to be cheaper because they are raised against multiple epitopes on your protein and are therefore less specific. Monoclonal are more expensive and take longer to produce, however they are specific to a single epitope. Monoclonal antibodies are most useful if you are trying to identify specific isoforms or region of a full-length protein, especially in tag fusion detection.

vsv-g-antibdy-if

Immunofluorescence staining of VSV-G fusion protein with VSV-G monoclonal antibody (Clone #14D2) in 293 cells with red and counterstained with DAPI.

A manufacturer may license their antibody to other companies, so do not buy three antibodies to test without making sure they are actually different. With common monoclonals that are supplied by many companies you might also consider shopping around for the best supplier of a clone.

Finally, remember you should feel free to contact the company supplier for advice any time. The suppliers really should be helpful, many pride themselves on their customer service, and if they are not you can remember that for next time you buy an antibody.

If you are looking for a detection tool to quantify or localize your VSV-G fusion proteins, we highly recommend you to try our featured monoclonal VSV-G Antibody risk free. This antibody with clone number 14D2 can specifically recognizes native and denatured forms of VSV-G fusion proteins, with which can meet your any requirements for Western Blot, Immunofluorescence and Immunoprecipitation assays.

Introduction to VSV-G antibodies from Sigma

One of the most frustrating things in the lab is spending good money on an antibody only to find out it doesn’t work well in your application. Now let start our VSV-G Monoclonal Antibody introduction from Sigma.

VSV-G antibodies from Sigma Sigma-Aldrich Corporation, started from 1975, is an American chemical, life science and biotechnology company headquartered in St. Louis and has operations in approximately 40 countries. In September 2014, the German company Merck KGaA announced that it would be acquiring Sigma-Aldrich to found a new Millipore-Sigma. Due to this combination, Merck-Millipore would supply these antibodies from 2016 in your region.VSV-G antibodies from Now Millipore-Sigma

Sigma (Now part of Millipore-Sigma) provide totally 2 VSV-G antibody, with one VSV-G polyclonal antibody and another VSV-G monoclonal antibody with clone #P5D4.

VSV-G antibody Clonality Host Applications
ANTI-VSV-G TAG antibody produced in rabbit Polyclonal Rabbit WB
Monoclonal Anti-VSV Glycoprotein antibody produced in mouse, clone P5D4 Monoclonal Mouse WB, IF, IP

The polyclonal VSV-G antibody can only be applied to WB assay with suggested 1:1000 dilution. So if you need VSV-G fusion protein localization analysis, you’d better choose another Monoclonal Anti-VSV Glycoprotein antibody. This monoclonal antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell epipolic and in the budding virus. It also can be used to pull down the VSV-G taged protein with IP assay.

As you know, one manufacturer may license their antibody to other companies, so do not buy three antibodies to test without making sure they are actually different. With common monoclonals that are supplied by many companies you might also consider shopping around for the best supplier of the target protein. Here, we put another high quality VSV-G Tag Mab from other vendor with Sigma VSV-G antibody together for your brief comparison.

Vendor Clone # Applications Price Yr 2017
Sigma VSV-G antibody P5D4 WB, IF, IP $340/100μl
$440/200μl
Abbkine Vs. Sigma VSV-G antibody 14D2 WB, IF, IP $90/50μl
$260/200μl

This Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) from Abbkine was affinity-purified from mouse ascites by affinity-chromatography using synthetic peptide immunogen containing the 15 carboxy-terminal amino acids (497-511) of VSV-G conjugated to KLH. So now you have two choice to get your VSV-G monoclonal antibody. If you’re willing to saving cost or only need small size, Abbkine would be yours. Or, get this Sigma VSV-G Antibody from EMD-Millipore now with good brand awareness.

VSV-G Tag Sequence

VSV-G, a vesicular stomatitis virus G (VSV-G) protein fragment, which is commonly used in biomedical research to pseudotype retroviral and lentiviral vectors, conveying the ability to transduce a broad stroll of mammalian cell sign with genes of interest.

As show below, recognized VSV-G epitope represents the amino acid sequence derived from the Vesicular Stomatitis viral glycoprotein.

VSV-G Tag Protein Sequence and Structure

 

The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods.

VSV-G Tag DNA Sequence

We cited VSV-G sequence information from Plasmid pCMV-VSV-G, which is from Dr. Bob Weinberg’s lab published in RNA 2003 Apr;9(4):493-501. In this vector, VSV-G tag sequence coding envelope protein for producing lentiviral and MuLV retroviral particles has been used in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).

VSV-G Sequence in pCMV-VSV-G Vector Map

DNA sequence coding for VSVG tag in pCMV-VSV-G vector is from 2936-2968 with total 33 nucleotides, TAT ACA GAC ATA GAG ATG AAC CGA CTT GGA AAG.

VSV-G Tag Amino Acid Sequence

VSV-G epitope tag is corresponding aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G), which is a 11 amino acid sequence YTDIEMNRLGK peptide derived from the Vesicular Stomatitis viral glycoprotein.

Sequence Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys
Chemical Name VSV-G Peptide
Canonical SMILES CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(CCSC)C
(=O)NC(CC(=O)N)C(=O)NC(CCCN=C(N)N)C(=O)
NC(CC(C)C)C(=O)NCC(=O)NC(CCCCN)C(=O)O)N
C(=O)C(CC(=O)O)NC(=O)C(C(C)O)NC(=O)C(CC1
=CC=C(C=C1)O)N
Formula C57H94N16O19S M.Wt 1334

If you are looking for a detection tool to quantify or localize your VSV-G fusion proteins, we highly recommend you to try our featured monoclonal VSV-G Antibody risk free. This antibody with clone number 14D2 can specifically recognizes native and denatured forms of VSV-G fusion proteins, with which can meet your any requirements for Western Blot, Immunofluorescence and Immunoprecipitation assays.