One of the most frustrating things in the lab is spending good money on an antibody only to find out it doesn’t work well in your application. Now let start our VSV-G Monoclonal Antibody introduction from Sigma.
Sigma-Aldrich Corporation, started from 1975, is an American chemical, life science and biotechnology company headquartered in St. Louis and has operations in approximately 40 countries. In September 2014, the German company Merck KGaA announced that it would be acquiring Sigma-Aldrich to found a new Millipore-Sigma. Due to this combination, Merck-Millipore would supply these antibodies from 2016 in your region.
Sigma (Now part of Millipore-Sigma) provide totally 2 VSV-G antibody, with one VSV-G polyclonal antibody and another VSV-G monoclonal antibody with clone #P5D4.
|ANTI-VSV-G TAG antibody produced in rabbit||Polyclonal||Rabbit||WB|
|Monoclonal Anti-VSV Glycoprotein antibody produced in mouse, clone P5D4||Monoclonal||Mouse||WB, IF, IP|
The polyclonal VSV-G antibody can only be applied to WB assay with suggested 1:1000 dilution. So if you need VSV-G fusion protein localization analysis, you’d better choose another Monoclonal Anti-VSV Glycoprotein antibody. This monoclonal antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell epipolic and in the budding virus. It also can be used to pull down the VSV-G taged protein with IP assay.
As you know, one manufacturer may license their antibody to other companies, so do not buy three antibodies to test without making sure they are actually different. With common monoclonals that are supplied by many companies you might also consider shopping around for the best supplier of the target protein. Here, we put another high quality VSV-G Tag Mab from other vendor with Sigma VSV-G antibody together for your brief comparison.
|Vendor||Clone #||Applications||Price Yr 2017|
|P5D4||WB, IF, IP||$340/100μl
|14D2||WB, IF, IP||$90/50μl
This Anti-VSV-G-Tag Mouse Monoclonal Antibody (14D2) from Abbkine was affinity-purified from mouse ascites by affinity-chromatography using synthetic peptide immunogen containing the 15 carboxy-terminal amino acids (497-511) of VSV-G conjugated to KLH. So now you have two choice to get your VSV-G monoclonal antibody. If you’re willing to saving cost or only need small size, Abbkine would be yours. Or, get this Sigma VSV-G Antibody from EMD-Millipore now with good brand awareness.